FastQCFastQC Report
Mon 8 Jan 2024
HNTFHDMXY_n01_MHC-052_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNTFHDMXY_n01_MHC-052_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27
Sequences flagged as poor quality0
Sequence length101
%GC35

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGTTAAATGGAACGGTAACAGGCTGTGCTACTGGACTGTAACTAGA13.7037037037037033No Hit
GACTGAAGTTATTAAAAAAAGAATCCATGAGGATCACTCCCCAAACTTTA13.7037037037037033No Hit
CATAAACCAATCATAACCCAATACAACATCGCATTAACGTAATATGATAA13.7037037037037033No Hit
AAACTTTGAACAAGTTTAAATAACAATTTAAAAAGAAAGAAAAATGAATG13.7037037037037033No Hit
TTCTAAACTAGACTAACCTACAATATCCTACAATATCCAACAAATAGATG13.7037037037037033No Hit
AGTAAATAAAAACAATCAAAAAAATTAAAGAAAAAAACAAAAAATTAAAA13.7037037037037033No Hit
ACATCAATCTTTTGAAGCAAAGCACTTACAAAAAATAAAAGAAAACAAAA13.7037037037037033No Hit
TTCAAAAAAACAGACCACCACAATCTCAATCACTCACACGCTGCACTGCA13.7037037037037033No Hit
CACAAAGATACTCCTATTAATCTAAACTAACACAAAATAAAAAAAAAGAA13.7037037037037033No Hit
GATTACACTCAAAATACTATCGGCACAGGAATATCAACCTGAATCGCTTA13.7037037037037033No Hit
TGCGGATCTTGGTACACACATACAAATATTACGAGTATCCAAAAAATAGC13.7037037037037033No Hit
CCCCGTAACTAAAAAAAATAAAAAAAAAATCGTATAGAAACAAAAAATAA13.7037037037037033No Hit
CTGCGCCCCGGGCCGGGGTCACTCACCGTCCTCGCTCTGGTTGTAGTAGC13.7037037037037033No Hit
CTCCAAAATTAAAACAATTTCCCACCATCATTCAATCCTAGAAAAAAAGG13.7037037037037033No Hit
TGCAGATTTCTTTTTTTAAAATTTTTTAGAGATAGGGACTCGCATTGTTT13.7037037037037033No Hit
CCTGTAGCTAGAACTAAAGGAACATGCCACAATGTCCTGCTAATATTTTG13.7037037037037033No Hit
CTTCATACATGTCACAACACTTCACCCCATTAACATGCTATCTCCAAAAG13.7037037037037033No Hit
CCCACAGCTTGCAAATAAATAAATGTATTCTGTTTATGGAATTAGGCTAA13.7037037037037033No Hit
CTCAAACAAGTATAAAGAGAAAACAAAAAAAAGATAATGAAATAATTAAA13.7037037037037033No Hit
CTCTGTCGCCCAGGCTGGAGTGCAGTGGCGGGATCTCGGCTCACTGCAAG13.7037037037037033No Hit
GCCCTCTGTGTGCAGCCTGCCGAGGCCACCGTCTAGCATGTCAGTCAGCG13.7037037037037033No Hit
GTAAATTTATTAATCGAAACTCTAATCAAATAAAATTAAATAAATGTATT13.7037037037037033No Hit
CCAATATAATATCTTATCACTCCTTCATTCAACGATAGACCTTACCGCAG13.7037037037037033No Hit
CTACCATAAACAATACTGGATATTCATCACACTGAGAATATCCTCACTGT13.7037037037037033No Hit
CCAACATCTCAGGGCTCTCTACCCTGCTGCCAAGCCAGAGGTTCAGCTTC13.7037037037037033No Hit
AGAAAAAATAAATTCTATTAAACATCAGCAGAAAAATGATAACAATAAAT13.7037037037037033No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT13.7037037037037033No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)