FastQCFastQC Report
Mon 8 Jan 2024
HNTFHDMXY_n01_MHC-041_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNTFHDMXY_n01_MHC-041_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTGCTCAGCACGTGGTTCATTGCCCAATGCGTCTGTTTGTGGCTCTCCA14.3478260869565215No Hit
TCAAGAACAAAATGCACTCGCTGGATATTTGAAAGATAATTAGAATCTTG14.3478260869565215No Hit
GTCAGGTCCAGCTCCAGGGGGCCCGGGGCCGAGCTGCACACCAGGGTCAG14.3478260869565215No Hit
CGCCAATATAAATCCTTTGAGATAATATACCTGACCAATGTACATACACA14.3478260869565215No Hit
CTTATAGACGTTCCGGAAGGGCATGACCGGTCCTGTCAGCTGGGTGGCCA14.3478260869565215No Hit
AAAATATCATTAAGGCTTATTTCGTTACATCTAAAATGAAAGCAGATTTT14.3478260869565215No Hit
CTGCCTTATAACTGGGGATTAATCACCAGGAAATAATAGAAAACCAAATG14.3478260869565215No Hit
CATTTAGAATACAAACACAAGTTGAGGAAGCTGCCTTTCCCGTTTGCCAA14.3478260869565215No Hit
GTGAAAAAATTGTATAACAACAAAATTTTGTAAAAAAATGATAATCAAAA14.3478260869565215No Hit
CCACACATTGTCAACAAACGGATGATCCATGAAGTCCGGGCCGCCGATGC14.3478260869565215No Hit
GGGGTGAGAGCACGGCTCATTTCAGATGTCTGCCCCAGCCAATGTCCCTG14.3478260869565215No Hit
CCGTAACTCACGTAAAACGCTACAATCCTGGTAATATAGTAGCTGCTCTG14.3478260869565215No Hit
GTCAGCTATTTAATTAGGTTCTTAAGACATTTAGTACACCAATTTGTGAG14.3478260869565215No Hit
GGAGGCAGAGACAGAGGCGGCGGCCCGGGGGATCCGGTACCCCCAAGGCA14.3478260869565215No Hit
CACCATACTTGCCTGTCCTGGACCATTAGAAATATAACAGAATTCTTAAG14.3478260869565215No Hit
AACAACAGTCTTGACTACCACACAAACAATATCAAAAACACTATCTATAT14.3478260869565215No Hit
CATCCACTCATTCACTGAATAAATATATCCTTAGCTCATACAATGAATAA14.3478260869565215No Hit
AGGATCTCTGAAAATATGAACTAATATGTCAAAAATAGTATTCTATCTCA14.3478260869565215No Hit
CAACACTCATTAAAACAACAAAAGATGCAGCTGCTCGAACTGTAGCCTGC14.3478260869565215No Hit
AGGAGGGAGCGTGAGGAGCAGGGGAGTTTTCTGTGCCCATTAGGCCGATG14.3478260869565215No Hit
CCCGTGAAAACCCCGGCCGCCACGGATGGGGTGAGCTTCCCGGTAGTGAC14.3478260869565215No Hit
CCGGCTATAGGAAAGGGATCATTACTTTTGCTCCATACTCTAAGATTTCC14.3478260869565215No Hit
CCCGGAGGGAAGTCAAGTCAGCCACATTGAGGGAGTCCTCTTGCAAGAGC14.3478260869565215No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)