FastQCFastQC Report
Mon 8 Jan 2024
HNTFHDMXY_n01_MHC-031_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNTFHDMXY_n01_MHC-031_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTATATTAGGCAACTCCAGCGATCCTGAGAGATCTTTCGAAAATAACCA18.333333333333332No Hit
CCTGGATGTGATAGTGTTTGTTCAGTTCTTCCATTGTAAAGTTACTTCCC18.333333333333332No Hit
CCCCGTTCGGCATGGCGAGGTGCGATTTCTGCGGCCCCCTCTTTCTCCGG18.333333333333332No Hit
CTCTATTACACTCAAAATACTATCGGCACAGGAATATCAACCTGTTTCCC18.333333333333332No Hit
CACATTTAGAAGTAAGTAAGTCAAGTTTTTCTTCAGTTTTACATTGTTCT18.333333333333332No Hit
CTCCTTAATGCTGGCAAAAAAAAAAAAAAAAGAAAAGTCACTTGCGTATG18.333333333333332No Hit
CTCGTGGAGCCCCTTCTATACGTTTGGGTCGCCGTAAGAGTCGCTATACG18.333333333333332No Hit
GTGAGTCTGTAATGACATCGCCACACTGGGCTGAGGTACGCTTTCTAGTT18.333333333333332No Hit
CCTGGCTGTTTCCCAGCCCCACCAACCAGGAGACATCACTCCTAGAGAAA18.333333333333332No Hit
ACGGCTTCGGATAATTCATAAAAACGAATTACTATTACTACAAAACAGGA18.333333333333332No Hit
CCTCACGGTACTTGTTTGCTATCGGTCTCCTACACATATTTAGCTTTATG18.333333333333332No Hit
TGTCAACTTCTGTAGCAAACCTCCACCAGAACCGAAGGCAATATTTTCAA18.333333333333332No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)