FastQCFastQC Report
Mon 8 Jan 2024
HNTFHDMXY_n01_MHC-028_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNTFHDMXY_n01_MHC-028_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCTCC23.8461538461538463No Hit
CCCCAAGCTACTCCTATTAATCGTAACTAAGCCAAAATAAGAAAAAAGAA23.8461538461538463No Hit
GTCGCTTCTTTGTTATAATTCCTTAAGTTAATAACTTTTTTCTTAAAAGG23.8461538461538463No Hit
CTGAATTGATTCAGCGAACCTCTTCTCCAAGTAAATTGATTCCAAGGTTT11.9230769230769231No Hit
GGTTAGAAGTTGGCAGCATGGGAAGGGGGAGGACCAGGTGGGGAATGGGG11.9230769230769231No Hit
CCCACTTGGAGTTATATTATTTGACAAACGAGCAAAATACAGCGAAGATA11.9230769230769231No Hit
CACGAAAAAGGTGATAGGATTATGAATGCATCCGTAGAGGAAGCACGTAT11.9230769230769231No Hit
CCCCCCTTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGC11.9230769230769231No Hit
GGGGCATTTGCGCTGTCGTCTATTTTGTTTAAATTGTTTTCATTGGAGTT11.9230769230769231No Hit
CCTATTACACTCAAAATACTATCGGCACAGGAATATCAACCTGTTTCCCT11.9230769230769231No Hit
CTTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTTCGG11.9230769230769231No Hit
GCCCACTTGGAGTTATATTATTTGACAAACGGGCAAAATACAAATAAAGA11.9230769230769231No Hit
CCCCTCGTTAGAGTGGGGACTAAGTGAAGAGCAGAGAATAAAAATTCCCA11.9230769230769231No Hit
CCCATCACGATATGTACTGATAAAGATTACCTACACTTTTCAGTGGAGGA11.9230769230769231No Hit
ATGCAGTCTCATAAACTTCATGTAAATTAATGACCCAAGGATTGTCTTGT11.9230769230769231No Hit
CTCACGAACAGCAGCTCACGAAAAAGGTGATAGGATTATGAATGCATCCG11.9230769230769231No Hit
CTCGGGTCCTACCAAATATGCTCTTACTCAATATAGCGTAATTGCTTACC11.9230769230769231No Hit
CAGAAACCGGGTTCCTGCCCTGTACTGTGTTCCCATGGCAACCTGAGCTT11.9230769230769231No Hit
TCGGAATCGTTCAGTTGACGTTGGTTCTTCAAATTATGGCTGCTCATGCT11.9230769230769231No Hit
CCCCCAAGCTACTCCTATTAATCGTAACTAAGCCAAAATAAGAAAAAAGA11.9230769230769231No Hit
GGACAGACTAGAAAAAGATCCTGGTAAGAACCTTTTATGAAAGTTAAAGT11.9230769230769231No Hit
GTTCATTTGTATATATGTTACCATCCCTATTACACTCAAAATACTATCGG11.9230769230769231No Hit
CTCGTCGCACACCTGCTTGAATTCCCGCTCGTAGAGTTCCCGGTCACGCT11.9230769230769231No Hit
CTATGAACGGGCACAAACGTGGGGATACACCCAAAGAGTCACGGGGCGGG11.9230769230769231No Hit
GCACCTTATCCAAGCGTTAGGAGCATCCCTCATCGCCAGTTCTGCTTACC11.9230769230769231No Hit
GAGGGGAGGGCCTGCCCCGGGGAGGGCACAGAGGCTGGGATGCTGGGGAC11.9230769230769231No Hit
CCCAAGGGGAATTAGCCCAATAAGCATACGTTCGCAATTTTTGCAATTAT11.9230769230769231No Hit
TTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCTCCAATCTCAAAG11.9230769230769231No Hit
GTCCTCTTCCTGCTTGTAGTGGCAGTCGTTTTGTTGCCCTTTGCCCTCCT11.9230769230769231No Hit
CCACTTGGAGTTATATTATTTGACAAACGAGCAAAATACAGCGAAGATAT11.9230769230769231No Hit
CTCCCTTGTTGTGTGCTTTTTTTTGTTTCCTCTTCTTTAACGTTTCCCTC11.9230769230769231No Hit
GGGGACGTAGTCAGCACAATCTGATGAATCATGCTTACTAGGCATTCCTC11.9230769230769231No Hit
GTCTACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAGC11.9230769230769231No Hit
GTATTGTTTAATCAGCATGGTTTCTGGCTTAAGAGGCGTTCAGCCATTAT11.9230769230769231No Hit
TCTGAAATGACTTTGAGGTGCTGGTGCTGTTTAAGGTGTGAGGCCTTAAC11.9230769230769231No Hit
CTCGGGTCCTACCAAATATGCTCTTACTCAATATAGCGTAATTGCTTATC11.9230769230769231No Hit
GGCCTGTCTTCACAATAGAGGGTCCACTAGACACAGTAAAGTGGGTGAAA11.9230769230769231No Hit
CTTCTTCCTCCTGAGGTCCTCCAGGTAGGCCTCCAGTAACCTCCTCTTCT11.9230769230769231No Hit
GTGAATTCGGGGACCTGCACAGACTGCAGGAATGTGGCCACAGCAGCTCG11.9230769230769231No Hit
TCTCCTATTAGTCTCTCCGTGTCCGTGAAGGCCACATACGATGGGGTGGT11.9230769230769231No Hit
AGAAAATCTAAGAATTTCACCACTCACATCAGAAAACAAAATAACCCAAG11.9230769230769231No Hit
TTGCAAAGATCCTGAAAGATGGGTCGTCCACATTTCACATGAGGAAACTG11.9230769230769231No Hit
CGAGGCAAAAAAGTCGTCTACAGTTTGTGCAATTCTTTTTTGTTGTTAAA11.9230769230769231No Hit
CTCCAATCTCAAAGAACACGACTCTAGAAGTAAAATTCCAAAAAATAGAA11.9230769230769231No Hit
TTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTTCGGA11.9230769230769231No Hit
GCTCCCTTCTCCACATGTACATACATGCAAGAACACACATTTTTGTAAAG11.9230769230769231No Hit
GTCGCATCTACTCACAATGGTAGCCCCGCTGATCCGTTCAAGTCTGTTCA11.9230769230769231No Hit
ACCTCGGCGGCCTCCGACTGGGCCCGCAGCCTGGACCCCTCACGCTCCCA11.9230769230769231No Hit
CTTAGTTCAAAGCCTCTCGGGTCCTACCAAATATGCTCTTACTCAATATA11.9230769230769231No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)