FastQCFastQC Report
Mon 8 Jan 2024
HNTFHDMXY_n01_MHC-020_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNTFHDMXY_n01_MHC-020_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTATTACACTCAAAATACTATCGGCACAGGAATATCAACCTGTTTCCA13.7037037037037033No Hit
CAGGAACTTTTATGTTTTTGATCTGATTTACAAACTGTACATGGGAAGCA13.7037037037037033No Hit
CCTCGTTCAAGATTAATAATTGCAATAATCTATCCCCATCACGATATGTA13.7037037037037033No Hit
CCTGATGCGGTTGTTGGTACGCTCATACAAGTATTAAGAGTATACAAAAA13.7037037037037033No Hit
GGTCCTTGCGCACCGTCCGCTTGAAGAAGCCCTTGCACCCCTCGCAGCTG13.7037037037037033No Hit
CTCCACGCTCTTCTCACCGCTGAAGTCGGTGTCCTGACTCTCTCTCCGCT13.7037037037037033No Hit
CAAATAAACACTTTTCAGCTATCCCTTAGGAGCGGCTAACTCTTGTCCAA13.7037037037037033No Hit
GAGGGTGTGAGGATGTACACGTTTGAGGGGTCAAGGACTAAACAAAAATC13.7037037037037033No Hit
CTTCCTCCTTTTCATTGCCTGCTCTCAGAGCAAGCTTGTCGATGAAGTAC13.7037037037037033No Hit
AATATTTTCTTCAAAGTAAGCAAATCAACCGAATTCAGTCCCACGTAAGA13.7037037037037033No Hit
CCCATCACGATATGTACTGATAAAGATTACCTACACTTTTCAGTGGAGGA13.7037037037037033No Hit
CGGCACCTTATCCAAGCGTTAGGAGCATCCCTCATCGCCAGTTCTGCTTA13.7037037037037033No Hit
AAGTAATATACCCATAAAAGCAAAGCTACAGCTAAACTCAAGAGCTGCGA13.7037037037037033No Hit
CCAAATATGCTCTTACTCAATATAGCGTAATTGCTTACAGGTATTGTATG13.7037037037037033No Hit
CCATCTCTATTACACTCAAAATACTATCGGCACACGAATATCAACCTGTT13.7037037037037033No Hit
GCCACGCTTTCACGTTTTCTACTTTTTCTGAAAATAGAAATCAAACAAGC13.7037037037037033No Hit
CCCTCCAATTGAGTTTTGGGAAGGTTTTAAATTCCCATCATTCCAATTAC13.7037037037037033No Hit
GTAGGGGGTGCGCGGCGTGCACGTACGTGGGTGCCAACACATGGGTTATT13.7037037037037033No Hit
GTGTAGAACTAGGCGCGTCGCTCTAAATGCGTACGCATTATGCCTCCTGC13.7037037037037033No Hit
GGCACCTTATCCAAGCGTTAGGAGCATCCCTCATCGCCAGTTCTGCTTAC13.7037037037037033No Hit
GTCGCTTCTTTGTTATAATTCCTTAAGTTAATAACTTTTTTCTTAAAAGG13.7037037037037033No Hit
GCGTTAGGAGCATCCCTCATCGCCAGTTCTGCTTACCAAAAATGGCCCAC13.7037037037037033No Hit
CAACAATAGATAAGTAAAACAACTTTAAAAGTAGTGGTATTTCACTGTCG13.7037037037037033No Hit
AAAGCTACTCCTAATAATCGTAACTCAGCCAAAAAAACAAAAAAGAACAA13.7037037037037033No Hit
CTACAAATATTTAGATTTATGTGAACTGTATCACACTAATAATTTATCCA13.7037037037037033No Hit
ACCTGCACAATGCATATGCTAATCGCCATAATTGCTAATAATTTTTTTTT13.7037037037037033No Hit
ATCGTTTCCTGTGTTGCAGGTCACTTCTTCAACAGGTGGCTGACTGCATC13.7037037037037033No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)