FastQCFastQC Report
Mon 8 Jan 2024
HNTFHDMXY_n01_MHC-013_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNTFHDMXY_n01_MHC-013_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19
Sequences flagged as poor quality0
Sequence length101
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCACATTTTAAAAAAAAAAATTACGAGAAAATGAACACTCAAAGCACATT15.263157894736842No Hit
GTCATCTATCACTTCAAATCCAATTATCCATCAATCCCATCCCAGGCACA15.263157894736842No Hit
GTCGTCTTTCCTGCAACAGAGGCATCCCTAGAGGCTGGGGCAGTCACCTG15.263157894736842No Hit
CAATACAATAACAAACTAACACACCAATTACCTCAACTCAGGAACAGATA15.263157894736842No Hit
CATAGATTTACAATTAAATAAGCAAAAAAAAGAACAAAAACATTAACAAT15.263157894736842No Hit
CATTTTTTTTTAATTTTATGCAAAAATTAAAACCATATGAAAAACTGTAA15.263157894736842No Hit
TTGGAATCCAACTTATAATACTCTCGGAAGCAATCTATACATAAACCACC15.263157894736842No Hit
CAAAAATAAAATCTTAATAAAATTACAACGCTTAAGCTCCTGAATATCCA15.263157894736842No Hit
CGGCCCCGGCACTTGACAATCATGTAACCCTTCTTCAGGTGGAACACGAG15.263157894736842No Hit
CCCCACCATAATCCATTATGGAAGTTCATAACTGTATCATCTTATAAAAT15.263157894736842No Hit
CCCAGCCATACATGTCACGCTCAAACTAGGACATCATTTCCAGAACATTT15.263157894736842No Hit
CCTGTATGGAAAGGGGCACTGAAGATCTGGCACAGAGAAACAAGGGGAGA15.263157894736842No Hit
GCTAAATAAAGACAAACCAGATTCAGTAGTGGATGACTTCATGGATCTGC15.263157894736842No Hit
GTCCCGTGCCATGTGTTCGAATTGAATTAGCAATATGCCGAATATTAATA15.263157894736842No Hit
CAAACATTAAAAAGACAACAACTCACACCACACACAAAAACCTGAAACAT15.263157894736842No Hit
GGCCACTAGACAATGGAATGGAATCATCAGCCTCACTGGATATAGAAGGT15.263157894736842No Hit
TCCTTTATGGTCTTATTTGTTTCTCCTCAGAAAAGACAAAAAGCTGAGCT15.263157894736842No Hit
AACAGTCTTTTCAAATTAAAACCACTCTGACAGACCACTGAAAAAAAATC15.263157894736842No Hit
CCCAAATGCCACAACACAATATAAAATCCGAAACAGAAAGAGCCTCCATC15.263157894736842No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)