FastQCFastQC Report
Mon 8 Jan 2024
HNTFHDMXY_n01_KHC-013_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNTFHDMXY_n01_KHC-013_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGAAAGTTTAGTAATTTTAAATTGTTTCTGTGAGCCCATGTAACACTGA12.5No Hit
GGCCCCACCATGTTCTTTAATTGGTTATTTGTGTAATCCCTGCTCTCCCT12.5No Hit
CACGACGAACATCCTTGACAGACACATTCTTGACATTGAAGCCCACATTG12.5No Hit
CTTCATGATCCTGTAAACTAGTCGTAGCAACTATTACTATCCCCATTTTA12.5No Hit
GTATGCCATCACACATTCCAAGCAAATAATCATTTTGTCAAGAAATAAGA12.5No Hit
TACGGCTTCGGATAATTCATAAAAACGAATTACTATTACTACAAAACAGG12.5No Hit
GTTGATTACACAGTAGTGGTGTTATTTTGTTGTCATAGGATGCAAATATC12.5No Hit
CTAAATTAAAAGAATAATCTCCTCAGAGAACTAATCTACTAGCCCGAAGC12.5No Hit
CTCCCTTTTTATTCTTCCAACTTAAAAAGCAGCCGTGTTTAGTTTGAATA12.5No Hit
AGAGGACACAGCTAGAAGGTGCCCTCTATGATAATGCAGGCCCTCACCAG12.5No Hit
CCCAACGATAATAATACACTATTTAAAAAGACCTAAGGCAATGAATTCCA12.5No Hit
CTTGATTTTAGCCCAGTCTTAGGTTTGTTTGAGCTGCCATAAAATACCAT12.5No Hit
AGCTATATTCTTGAAAAAAGATAAAAATTTTGTTTAATCTATATGTTATT12.5No Hit
CCCAAGGTCTACAGTTTCTTTTCTGTTGTGTCATCAGTGACAAAGAGTAA12.5No Hit
CCCACGTCTACATCCGTTCCTTTCTTTATCCACCCACAAAGCTGTAAAGC12.5No Hit
CTCCTTCAGGTCTTCTACAGAATCATTTTCATAGCCTGGTTCAGAGAAAG12.5No Hit
CTTAGACAAAACTGAGAATCAGTACTCAGTTTCATGACATTTGGTTAGCA12.5No Hit
GTTCTACAATCTCTTGTAAGGTATTAATATCTACTCCATCCCCTTGAATA12.5No Hit
CCTCTTACTAGAATGCCAAGAAAGTATTATGAAATCTAACGAAGTAAAGT12.5No Hit
GTCAGGGTTGATTCGGGAGGATCCTATTGGTGCGGGGGCTTTGTATGATT12.5No Hit
TAATCAGAGAGAGCTGGGTAAGGAGATCTAGAAAATAGTGGGAAAGTACT12.5No Hit
CGGCTTCGGATAATTCATAAAAACGAATTACTATTACTACAAAAAAGGAA12.5No Hit
CCCTTCTTAAAAAACTGGAATGTTGGCATGCATTTGACTTCACACTCTGA12.5No Hit
CTCTGATTTGGCCCTCACAGCAGCTGTTTTTTAGAAGAAACTGCACAGAG12.5No Hit
CCCCTTTCGGAATAACGGAGTCAATCAAAGCTCGGGTCTTATCCATAACT12.5No Hit
GCATGCCTGTAATTCCAGCTACTAGGGAGGCTGAGGCAGGAGAATTGCTG12.5No Hit
AAACTGAACCTTGGATTCAAAGACTATGCTCTTTATGATACCTAATTCTA12.5No Hit
CCCCCTGCGAAGCAACAATAAACTTTACATCTCTTTGGCAACAATAACTT12.5No Hit
CCTCTATTGCCATGTGCCTGGAATTATTATATGCTCATCACTTTATGAAG12.5No Hit
CTCCAATCTCAAAGAACACGACTCTAGAAGTAAAATTCCAAAAAATAGAA12.5No Hit
GGATGCGTAGGGATGGGAGGGCGATGAGGACTAGGATGATGGCGGGCAGG12.5No Hit
CCTCGGGTCCTACCAAATATGCTCTTAATCAATATAGCGTAAATGATTAC12.5No Hit
CTAAGTTTCCTGAACTGAAAAGGTAGGGATAATACCTCAAATACAGTATC12.5No Hit
GGACCAAGGCTGAAGAACTTCCTGTTTATGTACAGAATAATTATACTGTG12.5No Hit
CTCCGATCTGCACTGTTTTCACGCTGACAGTAGATTAGCATTTTCCACTT12.5No Hit
CATCCACCACTCAGTCCCCACTCAGTCCCCAACAGCTTGTCAGAGCATGG12.5No Hit
GCCTTTTCTACATCTATTGAGATAATCATTTGGTTTTTGTCTTTGGTTCT12.5No Hit
GCCCTATTTAACTAATGGCATTTTCACAAAAGCTCAACCAAAACGATTTC12.5No Hit
GCTCATACAAGTATTAAGAGTATACAAAAAAAGCTAAATGAAATAATTAA12.5No Hit
GGAGAAGGAGGAAAAGAGGGAGGAAGAGGAGTAGAAAGGAATAAAGAGTG12.5No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)