FastQCFastQC Report
Sat 19 Aug 2023
HHMVCDRX3_n02_S4_D28_PBMC_BCell.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHMVCDRX3_n02_S4_D28_PBMC_BCell.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8
Sequences flagged as poor quality0
Sequence length90
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTAACTCCTCCCAAGCTGGATCTGGGGTGTAAGAACTGTGACTTCAGAT112.5No Hit
CTGGAGCCTGGCGGACCCAACTCATGTAATTGTCACTGACGCTGAATCCG112.5No Hit
GTCTGGTGAGAATAGTGTTAATGTCATTAAGGAGAGAAGGAAGAGAAGTA112.5No Hit
AACCATTACAGGCGTCTAGCCTGACTTCTGTTGGTACCAATAGACATATT112.5No Hit
TTCATTATGCAGAAGGTATAGGGGTTAGTCGTTGCTATATTATGGTTGGT112.5No Hit
GGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCC112.5No Hit
CCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCCATTCGTGATGAGGA112.5No Hit
AGACCCTGTTGAGCTTGACTCTAGTCTGGCACGGTGAAGAGACATGAGAG112.5No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)