2025-02-03_SideA_Ildar
Run Name
150 + 150
Lane 1 (R1 + R2)
150 + 150
Lane 2 (R1 + R2)
860,975,640
Polonies
93.2%
Q30
98.6%
Index Assignment
254.410
Assigned Yield (Gb)
6 + 0
Lane 1 (I1 + I2)
6 + 0
Lane 2 (I1 + I2)
N/A
Unexpected Pairs
Index Percentiles
Percentile Sample I1 I2 Polonies % Polonies
5th PhiX GCACAT 4,989,211 0.5795
50th IG25withDOX_rep2 ACTTGA 17,620,286 2.0465
95th IG25noDOX_rep2 CGATGT 24,998,070 2.9035
All Indices
# Sample I1 I2 Polonies % Polonies
1 IG13noDOX_rep2 TATAAT 26,261,519 3.0502
2 IG13noDOX_rep1 TACAGC 25,128,594 2.9186
3 IG25noDOX_rep2 CGATGT 24,998,070 2.9035
4 IG26noDOX_rep3 GCCAAT 24,739,977 2.8735
5 IG26noDOX_rep2 ACAGTG 24,275,927 2.8196
6 IG26noDOX_rep1 TGACCA 23,840,676 2.7690
7 IG15noDOX_rep2 ATGAGC 22,890,812 2.6587
8 IG13noDOX_rep3 TCATTC 22,720,105 2.6389
9 IG13withDOX_rep1 TCCCGA 22,595,012 2.6243
10 IG18withDOX_rep1 CTCAGA 22,573,225 2.6218
11 IG18noDOX_rep1 CGGAAT 21,960,393 2.5506
12 IG18noDOX_rep2 CTAGCT 21,838,468 2.5365
13 IG26withDOX_rep3 CTTGTA 21,229,800 2.4658
14 IG15noDOX_rep3 ATTCCT 21,055,789 2.4456
15 IG15noDOX_rep1 ACTGAT 20,998,545 2.4389
16 IG26withDOX_rep1 TAGCTT 20,451,302 2.3754
17 IG18noDOX_rep3 CTATAC 20,229,873 2.3496
18 IG13withDOX_rep3 TCGGCA 20,196,045 2.3457
19 IG13withDOX_rep2 TCGAAG 19,440,166 2.2579
20 IG25noDOX_rep3 TTAGGC 18,608,203 2.1613
21 IG18withDOX_rep2 GACGAC 18,474,572 2.1458
22 IG19withDOX_rep3 CCAACA 18,168,845 2.1103
23 IG15withDOX_rep1 CAAAAG 18,066,431 2.0984
24 IG19withDOX_rep1 CATGGC 18,049,572 2.0964
25 IG18withDOX_rep3 TAATCG 17,973,299 2.0876
26 IG25withDOX_rep3 GATCAG 17,726,869 2.0589
27 IG25withDOX_rep2 ACTTGA 17,620,286 2.0465
28 IG25withDOX_rep1 CAGATC 17,565,924 2.0402
29 IG26withDOX_rep2 GGCTAC 16,969,065 1.9709
30 IG19withDOX_rep2 CATTTT 16,262,717 1.8889
31 IG27withDOX_rep1 CCGTCC 15,603,535 1.8123
32 IG27noDOX_rep2 AGTTCC 15,048,085 1.7478
33 IG28withDOX_rep2 GAGTGG 14,847,722 1.7245
34 IG27noDOX_rep1 AGTCAA 14,560,336 1.6911
35 IG15withDOX_rep2 CAACTA 14,497,957 1.6839
36 IG27noDOX_rep3 ATGTCA 14,069,536 1.6341
37 IG19noDOX_rep2 CACTCA 13,287,023 1.5433
38 IG28noDOX_rep2 GTGGCC 13,247,118 1.5386
39 IG28noDOX_rep1 GTGAAA 12,036,705 1.3980
40 IG19noDOX_rep3 CAGGCG 11,909,536 1.3833
41 IG27withDOX_rep3 GTCCGC 11,495,136 1.3351
42 IG28noDOX_rep3 GTTTCG 11,393,613 1.3233
43 IG28withDOX_rep1 CGTACG 10,937,428 1.2704
44 IG27withDOX_rep2 GTAGAG 10,227,947 1.1879
45 IG25noDOX_rep1 ATCACG 8,942,716 1.0387
46 IG28withDOX_rep3 GGTAGC 8,468,882 0.9836
47 PhiX ATGTCG 5,726,672 0.6651
48 PhiX CACAGA 5,684,017 0.6602
49 PhiX TGTGTC 5,199,302 0.6039
50 PhiX GCACAT 4,989,211 0.5795
51 IG19noDOX_rep1 CACGAT 3,911,745 0.4543
52 IG15withDOX_rep3 CACCGG 23,916 0.0028
Top Unassigned Sequences
# I1 I2 Polonies % Polonies
1 CAACAA 293,501 0.0341
2 CAATAA 153,611 0.0178
3 ATGGCA 146,969 0.0171
4 AAAAGA 129,473 0.0150
5 ATTTTA 117,543 0.0137
6 CACTAA 111,059 0.0129
7 CCAAAA 98,497 0.0114
8 CATTTA 96,380 0.0112
9 ACTCAA 84,540 0.0098
10 AGGCGA 83,315 0.0097
11 CATGGA 76,872 0.0089
12 TAGCCT 76,197 0.0089
13 ATGAGA 63,129 0.0073
14 CATGCA 55,749 0.0065
15 CAAAGA 50,488 0.0059
16 AACTAA 45,448 0.0053
17 CATCAA 42,504 0.0049
18 CAGGGA 36,740 0.0043
19 ATTTCG 32,140 0.0037
20 CAGGCA 26,818 0.0031
21 GTAAAG 26,714 0.0031
22 GACAAC 25,089 0.0029
23 CAAAAA 24,106 0.0028
24 ATAGCA 22,278 0.0026
25 CAGATA 21,546 0.0025
26 GAGTGA 20,391 0.0024
27 TCTACA 17,270 0.0020
28 CGAAAT 15,143 0.0018
29 TAGCAT 14,377 0.0017
30 TAGCGT 13,026 0.0015
31 CCCAGA 11,586 0.0013
32 TCGACA 10,979 0.0013
33 GGTAGA 10,014 0.0012
34 ACGATA 7,900 0.0009
43.2
Mean Q
97.2%
Q30
90.9%
Q40
N/A
Reads eliminated
150.0
Mean read length
N/A
Trim type
N/A
Min read length