FastQCFastQC Report
Thu 19 Jul 2018
HY7YMBGX5_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHY7YMBGX5_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12966450
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1797561.3863162237929427No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCG633700.064.425721
GAGGATC649950.064.2786911
CGGCCGA653600.064.213046
CGAGGAT653400.063.95511210
GTCGCGG648150.063.803272
GGCCGAG660400.063.5518577
TCGCGGC653050.063.5440373
CGCGGCC666900.063.0086754
AGGATCA240350.062.8371212
GCGGCCG671300.062.6837355
CCGAGGA670650.062.4405489
AGGATCC149050.062.0503412
AGGATCG53050.060.4211612
GCCGAGG713550.058.8581358
GGATCAT98200.058.72522713
AGGATCT250700.057.71652612
GGATCAA94100.057.1933713
GGATCCC24000.054.96789613
GGATCCA72900.054.91329213
GGATCTG45250.054.6738713
Produced by FastQC (version 0.11.5)