FastQCFastQC Report
Thu 19 Jul 2018
HY7YMBGX5_n01_DAM_GR_bZIP1_R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHY7YMBGX5_n01_DAM_GR_bZIP1_R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28410188
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGGA343480.1209002911209176No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGGATC9068000.065.1613811
AGGATCT3260750.064.8632112
AGGATCA2949700.064.7336912
AGGATCC2156600.064.7329512
CGAGGAT9145500.064.7097210
AGGATCG763800.064.31399512
CGGCCGA9272350.064.2668156
GGATCCA923100.064.2407413
GGATCTC777200.064.2005413
GCGGCCG9295750.064.095365
CCGAGGA9266800.064.0844969
GGCCGAG9298600.064.081137
GCCGAGG9299200.064.076938
CGCGGCC9292000.064.054944
GGTCGCG9264000.064.005751
GGATCAT1064350.063.9128413
TCGCGGC9288150.063.8891833
GTCGCGG9293500.063.848222
GGATCTA610200.063.8074213
GGATCAC463250.063.77834713
Produced by FastQC (version 0.11.5)