FastQCFastQC Report
Thu 19 Jul 2018
HY7YMBGX5_n01_DAM_GR_bZIP1_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHY7YMBGX5_n01_DAM_GR_bZIP1_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19272421
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG267280.13868522278545076No Hit
GGTCGCGGCCGAGGATCAAGTTCACCACCGCATGTCGGTACGCTCCAGGC229800.11923774392433623No Hit
GGTCGCGGCCGAGGATCCATGCTTTCCAACGAAGCACGCCCATCCAACCT209680.10879795537882865No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGGATC7560050.064.52080511
AGGATCT2580650.064.4677112
AGGATCG592600.064.36719512
GGATCTC672400.064.3642213
AGGATCA2592700.064.3179212
CGAGGAT7605400.064.1943210
CGGCCGA7648300.064.103466
GGATCCA775000.064.1027513
AGGATCC1821450.064.0774412
GCGGCCG7658050.063.994765
GGATCTT999050.063.94605613
GGTCGCG7636400.063.9400831
CGCGGCC7657600.063.9372754
GGATCAT915250.063.93129713
CCGAGGA7650500.063.925229
GGCCGAG7677900.063.867737
GGATCTA464950.063.86123713
GCCGAGG7680600.063.84598
GTCGCGG7664300.063.7570422
TCGCGGC7667000.063.7321433
Produced by FastQC (version 0.11.5)