FastQCFastQC Report
Thu 19 Jul 2018
HY7YMBGX5_n01_DAM_GR_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHY7YMBGX5_n01_DAM_GR_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45118740
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGGA2681840.5943960314494597No Hit
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGA1735930.3847470031299633No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGGATC8865500.063.42122711
AGGATCC2138150.063.28627412
AGGATCG729650.063.0646512
AGGATCA2986300.063.0248912
AGGATCT3068800.062.87586212
GGATCCA896100.062.8214413
CGAGGAT8994700.062.6259410
GGATCTC775200.062.51062413
GGATCCC346450.062.43979613
GGATCAC479650.062.4294713
GGATCAT1086050.062.34900713
GGATCTG522500.061.91800313
GGATCTA587800.061.88657413
GGATCGT199900.061.7848713
GGATCAA1065850.061.5966213
GGATCGC180200.061.4310213
GGATCAG410600.061.00358213
GGATCCG324150.060.96983713
GGATCTT1245950.060.89784613
CGGCCGA9347450.060.6144456
Produced by FastQC (version 0.11.5)