FastQCFastQC Report
Thu 19 Jul 2018
HY7YMBGX5_n01_DAM_GR_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHY7YMBGX5_n01_DAM_GR_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40543584
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGGA625960.1543918761597396No Hit
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGA439150.10831553520280791No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGGATC12505400.064.6045611
AGGATCT4471750.064.5300712
AGGATCG794950.064.48331512
AGGATCA4334700.064.37907412
AGGATCC2942600.064.3577412
GGATCTC1154000.064.2756713
GGATCCA1280200.064.2736213
CGAGGAT12608950.064.1313410
GGATCTT1759750.064.1284713
GGATCTA852450.064.11626413
GGATCCC447050.064.0300213
GGATCAT1650700.063.94654513
GGATCCG346250.063.93081713
GGATCTG736300.063.89706813
CGGCCGA12709800.063.8126266
GGATCAA1530450.063.80971513
GCGGCCG12728550.063.715565
CGCGGCC12730400.063.6535154
GGTCGCG12689350.063.6426541
CCGAGGA12734600.063.5958569
Produced by FastQC (version 0.11.5)