FastQCFastQC Report
Thu 19 Jul 2018
HY7YMBGX5_n01_C_AND_R_EV_HA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHY7YMBGX5_n01_C_AND_R_EV_HA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35356612
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCG23800.023.8255921
GTCGCGG25600.022.4223582
CGGCCGA25600.022.285516
GCTAGGA183400.012.51823860
TAACGCG163550.011.9833158
GAGCTAG190900.011.89811258
CGAATGA192350.011.86350840
AGCTAGG192500.011.79920259
GGGCGTC136050.011.47439234
AGATTCG202250.011.47397935
ACGCGAA174000.011.40440260
AGGTCTG206900.011.36799056
CGCGAAT174100.011.35767961
GAAGGTC205400.011.3488014
AACGCGA174350.011.32128859
TCGCCCC117350.010.85567910
AGCCATC209700.010.79839769
CGCCCCG117300.010.77076711
TGACTTA214200.010.71841144
AAGGTCT223150.010.5715625
Produced by FastQC (version 0.11.5)