FastQCFastQC Report
Thu 19 Jul 2018
HY7YMBGX5_n01_C_AND_R_EV_H3K27AC.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHY7YMBGX5_n01_C_AND_R_EV_H3K27AC.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43548536
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGCAG63800.014.1002731
CCTAGGC74450.013.39798240
CCATGCG47000.012.58486639
CCGGGCC36400.012.40348170
CGCCTAG51700.012.18560838
AGGGGCG32800.011.7390781
CCCGCGT30550.011.68510859
GGGCGTG61350.010.8958510
AGGCCGT38150.010.8268611
AGGGTTT532200.010.7865881
GCGGGGG18550.010.75512920
GCGTGCT59450.010.71430112
GTCCCGA59950.010.68399348
GCGATCC57350.010.679769543
GGCGCCC16600.010.54159357
GTCGCGA37200.010.25501564
GCCCGCG36800.010.176084558
TGTCCCG60600.010.16511447
CGCACGG22450.010.13428624
TACCCGG46700.010.04247367
Produced by FastQC (version 0.11.5)