FastQCFastQC Report
Thu 19 Jul 2018
HY7YMBGX5_n01_C_AND_R_EV_GR.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHY7YMBGX5_n01_C_AND_R_EV_GR.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22920558
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGAAGGTCTGAAGAGCCAAGAGTTATTTGATGAGATTCGAATGACTTA283280.12359210452031752No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTAGGA134150.016.07067360
AGCTAGG136100.015.96899159
GAGCTAG134600.015.91293858
CGTATGC13150.015.43755246
CGAATGA139400.015.31549240
AGCCATC144400.014.83333169
AGGTCTG146600.014.7304196
TATGCCG14500.014.482903548
GAAGGTC150000.014.420024
AGATTCG149800.014.2295535
TCGAATG151200.014.1896839
TTATATC154150.014.14545648
TGACTTA152450.014.14202244
GTATGCC14600.013.90434247
GATTCGA158000.013.73422636
ACTTATA158200.013.717072546
AAGGTCT158900.013.6564055
TAGGAAA160050.013.6450162
GTTCCGT21950.013.55408135
GAGTTAT164950.013.28335322
Produced by FastQC (version 0.11.5)