FastQCFastQC Report
Fri 27 Jul 2018
HWLMWBGX5_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWLMWBGX5_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24318181
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG557170.22911664322261605No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC428150.056.5261513
CGTCTGA423600.055.4573516
ACGTCTG427500.055.26214615
CACGTCT436500.054.7479314
GCACACG458200.054.58983611
CACACGT456000.054.57773212
ATCGGAA549150.052.699842
CGGAAGA535600.052.621094
TCGGAAG543150.052.3529973
GATCGGA552950.052.250241
GAACTCC477350.047.8144621
AGCACAC556500.046.3931710
CTGAACT501550.046.38503319
AGAGCAC580650.045.6568648
TCTGAAC530200.044.30088818
GAGCACA602100.043.4898269
GTCTGAA555250.042.7372217
TGAACTC546100.042.56984720
GAAGAGC645200.042.152866
AACTCCA538700.041.75314722
Produced by FastQC (version 0.11.5)