FastQCFastQC Report
Fri 27 Jul 2018
HWLMWBGX5_n01_mb119.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWLMWBGX5_n01_mb119.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33839792
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCTCGTATGC1478050.4367786894198404TruSeq Adapter, Index 14 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATGTCGTATGC395640.11691561224726203TruSeq Adapter, Index 14 (97% over 41bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG235050.061.87313543
CTCGTAT191500.061.15769242
CGTATGC252600.061.00899544
TCTCGTA193100.060.4701341
ATGCCGT247650.059.44261647
TATGCCG264700.058.7481946
TGCCGTC245000.058.6279948
ATCTCGT213000.057.4829840
GCCGTCT228400.057.44942549
CCGTCTT234750.057.3567950
TCGCGTA16950.055.3429441
CGTCTTC228750.055.25054651
CATCTCG225450.054.99286739
CGCGTAT18650.052.55004542
GTCGTAT63050.052.12840342
GTATGCC303700.051.6648845
CGTCTGA420250.050.9653916
TGTCGTA66000.049.37451641
ACGTCTG434650.049.30896415
CCGTCGT25600.049.08205450
Produced by FastQC (version 0.11.5)