FastQCFastQC Report
Fri 27 Jul 2018
HWLMWBGX5_n01_mb118.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWLMWBGX5_n01_mb118.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36370130
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTCGTATGC2528040.6950868748613216TruSeq Adapter, Index 13 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT316400.061.82919742
TCGTATG326200.061.7418343
TCTCGTA318700.061.6797841
CGTATGC329250.061.26387844
AATCTCG328200.060.61096239
ATGCCGT328950.060.38158447
TATGCCG336150.059.84900746
TGCCGTC330750.059.2696648
ATCTCGT337300.058.8290840
GCCGTCT321800.058.78625549
CCGTCTT324850.058.66503550
CGTCTTC325400.056.7587351
GTATGCC383000.053.13097445
CGTCTGA455850.049.33444616
ACGTCTG474750.047.4586215
ACACGTC494650.045.68345313
GTCAAAT477900.044.91612635
CACGTCT505450.044.63833214
GCTTGAA407350.044.4883359
CAGTCAA500400.044.0244933
Produced by FastQC (version 0.11.5)