FastQCFastQC Report
Fri 27 Jul 2018
HWLMWBGX5_n01_mb117.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWLMWBGX5_n01_mb117.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31687740
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC5736011.8101669604711477TruSeq Adapter, Index 11 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT677050.064.3067642
TCGTATG683250.064.30170443
TCTCGTA675050.064.0674341
CGTATGC689850.063.92863544
ACGGCTA718450.063.7545132
TATGCCG692750.063.65445746
ATGCCGT689400.063.5774347
CGGCTAC717850.063.20879433
TGCCGTC690600.062.9146448
GCCGTCT673750.062.6598249
ATCTCGT693350.062.53334840
CCGTCTT679750.062.3746850
CATCTCG694500.062.33008639
TCACGGC736450.062.29377430
GTCACGG745550.061.74394229
CGTCTTC675200.061.4778751
AGTCACG756750.061.11220628
CACGGCT753200.060.87380631
GTATGCC738150.059.96233745
CGTCTGA799750.058.64743816
Produced by FastQC (version 0.11.5)