FastQCFastQC Report
Fri 27 Jul 2018
HWLMWBGX5_n01_mb116.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWLMWBGX5_n01_mb116.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32839076
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC765800.23319779155783799TruSeq Adapter, Index 10 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGCGTATGC447860.1363802075308087TruSeq Adapter, Index 10 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGGGGTTGG401430.12224156367858828TruSeq Adapter, Index 10 (97% over 42bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGCG215250.066.0552439
CTTATCG578750.065.350937
TCGCGTA88200.064.6866941
ATCGCGT140350.064.5683340
TTATCGG355900.064.4210438
TATCGGG349700.064.2013139
CGCGTAT87600.063.77122542
TTATCGC229200.063.73063338
CGTCTGA979450.062.2362216
GCTTATC930850.062.23323436
ACGTCTG990700.061.55374515
ACACGTC997800.061.2660113
TAGCTTA983600.060.6646934
ACTAGCT993500.060.47773732
CACGTCT1010750.060.43640514
GTCACTA1002300.060.16154529
ATCGGGT168050.059.8824140
AGCTTAT988550.059.826935
ATCGGGG207350.059.8090740
CACTAGC1006900.059.76648331
Produced by FastQC (version 0.11.5)