FastQCFastQC Report
Fri 27 Jul 2018
HWLMWBGX5_n01_mb115.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWLMWBGX5_n01_mb115.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31588640
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC21784346.896257641987752TruSeq Adapter, Index 9 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATATCGTATGC343350.10869413814586511TruSeq Adapter, Index 9 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT2482400.065.358242
TCTCGTA2463400.065.3424741
TCGTATG2552700.065.2621343
GATCTCG2446800.065.20254539
CGTATGC2559400.065.199844
CGATCAG2559550.065.19124633
ACGATCA2572700.065.10046432
TATGCCG2560950.065.08813546
ATGCCGT2558250.065.087747
ATCTCGT2456300.064.9949440
GTCACGA2582450.064.92847429
CACGATC2578800.064.9103331
GCCGTCT2537350.064.8996549
TGCCGTC2557600.064.8852948
CCGTCTT2540350.064.85255450
TCACGAT2583900.064.8457530
CGTCTGA2634500.064.73144516
CGTCTTC2537600.064.5959551
ACACGTC2660500.064.58707413
AGTCACG2606850.064.49077628
Produced by FastQC (version 0.11.5)