FastQCFastQC Report
Fri 27 Jul 2018
HWLMWBGX5_n01_mb114.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWLMWBGX5_n01_mb114.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36020446
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAATCG211050.060.1731137
AATCGCG80700.056.951139
AATCGGG137600.056.65166539
TCGCGTA23600.056.2117741
GAATCGG141650.055.9468438
ATCGCGT43250.055.43807240
CGCGTAT23800.054.70938542
GAATCGC91100.052.06389238
ATCGCGG42550.051.74337440
ATCGGGG105100.049.0906140
CGTCTGA416900.047.4119816
ATCGGGT56800.046.5883340
ACGTCTG431450.045.86136615
AATCTCG69200.044.56364439
TTGAATC409400.044.47876736
ACACGTC454500.043.7277613
TCGCGTT21800.043.03069341
CACGTCT463050.042.89791514
GTCACAC465050.041.95889729
CGCGGAT24900.040.62508842
Produced by FastQC (version 0.11.5)