FastQCFastQC Report
Fri 27 Jul 2018
HWLMWBGX5_n01_mb113.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWLMWBGX5_n01_mb113.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28101666
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC642830.22875156227392357TruSeq Adapter, Index 3 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTA20300.058.96861641
CGCGTAT20650.057.29043642
TCTCGTA93450.057.15656341
TCGTATG88850.057.1206243
CTCGTAT93100.057.03236442
ATCGCGT28900.055.95479240
CGTATGC102150.055.9531844
ATGCCGT98650.054.14037747
TATGCCG107800.054.1233346
CATCGCG41050.052.35503439
TGCCGTC103650.050.17808548
CATCTCG126950.049.6850139
CGTCTGA243250.049.1614916
ATCTCGT122700.049.12264640
CCGTCTT101250.049.017550
ACGTCTG247950.048.32798815
ACACGTC252800.047.34467313
GCCGTCT99100.046.93731349
CTTAGGC259200.044.93328533
CACGTCT267550.044.73520314
Produced by FastQC (version 0.11.5)