FastQCFastQC Report
Fri 27 Jul 2018
HWLMWBGX5_n01_mb112.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWLMWBGX5_n01_mb112.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35786555
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC12019323.358613311619406TruSeq Adapter, Index 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT1377000.066.1321842
TCTCGTA1360150.066.0847841
TCGTATG1423700.066.0569243
CGTATGC1430850.065.90365644
CGATCTC1346350.065.8908238
ACGATCT1347600.065.79462437
CACGATC1350650.065.7436936
ATGCCGT1429350.065.7314747
TATGCCG1432850.065.7128546
TCACGAT1402150.065.4844735
ATCACGA1425750.065.4190834
TGCCGTC1431400.065.2975548
CCGTCTT1417150.065.2502750
GCCGTCT1416350.065.2329449
GATCTCG1365450.065.1604939
CATCACG1453900.064.9423933
CGTCTTC1411700.064.8552651
ATCTCGT1376750.064.7058640
GTATGCC1494150.063.1763445
CGTCTGA1542950.062.65892816
Produced by FastQC (version 0.11.5)