FastQCFastQC Report
Fri 27 Jul 2018
HWLMWBGX5_n01_mb111.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWLMWBGX5_n01_mb111.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38763156
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC3510280.905571259471236TruSeq Adapter, Index 12 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGTCGTATGC577770.14905133111452534TruSeq Adapter, Index 12 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA415650.064.89332641
TCGTATG469900.064.8793543
CTCGTAT412950.064.8423642
CGTATGC480400.063.86799244
ATGCCGT455600.063.8159347
AATCTCG446450.063.49165739
TATGCCG481950.063.254546
TGCCGTC459450.062.328748
CCGTCTT450300.061.86224450
GCCGTCT440050.061.41005749
CGTCTTC435850.060.09085551
ATCTCGT466800.060.07771740
GTCGTAT86000.057.99777642
GTATGCC555950.055.77303345
CGTCTGA712550.055.70130516
GTAATCT523400.055.62894437
TGTCGTA91950.055.0446841
ACGTCTG724150.054.86177415
ACACGTC737700.053.89635513
CCTTGTA725950.053.53188333
Produced by FastQC (version 0.11.5)