FastQCFastQC Report
Fri 27 Jul 2018
HWLMWBGX5_n01_mb110.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWLMWBGX5_n01_mb110.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28427481
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC645760.2271604719391071TruSeq Adapter, Index 7 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTA38100.063.7569941
CATCGCG93500.063.080239
ATCGCGT64350.062.98734340
CGCGTAT38500.062.6395742
ATCATCG177600.059.79861537
TCTCGTA101600.059.22096341
CGTATGC111150.058.56981744
TCGTATG97150.057.9694143
CTCGTAT103100.057.81575842
TATGCCG125150.057.21914346
CGTCTGA418850.056.959716
ATCGCGG32600.056.36831340
ATGCCGT107100.055.94722747
ATCTCGT137950.055.54146640
ACGTCTG430000.055.44193315
CATCTCG156950.055.0635939
TCATCGC109700.054.7864338
TCGCGTT27650.054.68672641
ACACGTC436650.054.63659313
TGCCGTC110550.054.35925748
Produced by FastQC (version 0.11.5)