FastQCFastQC Report
Fri 27 Jul 2018
HWLMWBGX5_n01_mb109.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWLMWBGX5_n01_mb109.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31792201
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC2278710.716751256070632TruSeq Adapter, Index 6 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA271150.063.86023341
CTCGTAT269300.063.7397342
TCGTATG268100.063.58073443
CGTATGC271400.063.19316544
ATGCCGT267400.062.95743647
CGCCAAT290100.062.7371733
TATCTCG282100.062.4128539
TATGCCG278000.061.5399746
ATCTCGT288250.061.1287540
ACGCCAA298850.061.08656732
TGCCGTC273900.060.95205348
GCCGTCT267900.060.39669449
CCGTCTT270750.060.31755450
GTCACGC306450.059.7898829
TCACGCC313150.058.32160630
CGTCTTC271500.058.2816951
CACGCCA320550.056.89663331
AGTCACG327200.056.46858228
GTATGCC315400.054.7754245
GCCAATA348800.052.35921534
Produced by FastQC (version 0.11.5)