FastQCFastQC Report
Fri 27 Jul 2018
HWLMWBGX5_n01_mb108.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWLMWBGX5_n01_mb108.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31120851
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGTCGTATGC1128080.36248366087418366TruSeq Adapter, Index 5 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC1062720.34148166449561423TruSeq Adapter, Index 5 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC290950.064.19137644
TCGTATG254200.064.13753543
ATGCCGT269800.063.2922747
GTCGTAT144550.063.00555842
TATGCCG300300.062.4590146
TCGCGTA21850.062.1542341
TGCCGTC270500.062.08043748
TCTCGTA137950.062.06191641
CTCGTAT137750.061.7707742
TGTCGTA148450.061.6569341
GATGTCG174400.061.59612339
CCGTCTT262900.061.3725650
CGCGTAT21750.061.15247342
GCCGTCT250050.060.99931349
CGTCTGA510050.060.4080616
ACGTCTG517650.059.59495515
CGTCTTC246250.059.50989551
ATGTCGT169950.058.8824940
GATCTCG160100.058.70240439
ACACGTC527900.058.5433313
Produced by FastQC (version 0.11.5)