FastQCFastQC Report
Fri 27 Jul 2018
HWLMWBGX5_n01_mb107.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWLMWBGX5_n01_mb107.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29387780
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC9978783.395554206544353TruSeq Adapter, Index 4 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT1144150.066.2751342
TCTCGTA1142200.066.18635641
TCGTATG1178850.066.15604443
ATGCCGT1164050.066.0881547
CGTATGC1180550.066.0401844
TGCCGTC1155550.065.94102548
TATGCCG1177950.065.90346
GCCGTCT1131000.065.8465249
AATCTCG1149650.065.7625139
CCGTCTT1138000.065.715250
ATCTCGT1158600.065.4069240
CGTCTTC1123100.065.3528551
GTATGCC1221200.063.94664845
ACCAATC1211000.062.4661436
CGTCTGA1317400.062.23639716
GACCAAT1263450.062.07356635
ACGTCTG1329500.061.7040915
GCTTGAA1169000.061.6223259
CCAATCT1232050.061.35196337
CAATCTC1238250.061.06948538
Produced by FastQC (version 0.11.5)