FastQCFastQC Report
Fri 27 Jul 2018
HWLMWBGX5_n01_mb106.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWLMWBGX5_n01_mb106.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34761882
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC1545480.44459042810167754TruSeq Adapter, Index 2 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGCGTATGC477910.1374810489259471TruSeq Adapter, Index 2 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGCG140750.065.43017639
CGATGTA582700.065.1061734
TCGCGTA76400.065.010441
ACCGATG590050.064.5207232
CCGATGT589100.064.4888333
CACCGAT593450.064.2684931
CGCGTAT77100.063.82961342
ATCGCGT108550.063.26546540
GTATCGC150600.062.91885438
TCTCGTA214000.062.59483341
GTCACCG611350.062.5421629
CTCGTAT212150.062.5298642
TCACCGA611850.062.50375430
TGTATCG251100.062.4390437
CGTATGC235200.062.38267544
TATCTCG272650.062.38023839
TCGTATG195650.062.18561643
ATGCCGT224300.061.09016447
TATGCCG264200.060.939446
ATCTCGT259700.059.9771240
Produced by FastQC (version 0.11.5)