FastQCFastQC Report
Tue 3 Jul 2018
HWLKJBGX5_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWLKJBGX5_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72528817
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGCGCGCGGCGCGGCGC2465770.3399710765998017No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGCGCGCGGCGC2292150.31603300519847166No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGCGCGCGGCG2078260.286542657934156No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGCGCGCGGCGCGGCGCG2052930.2830502529773786No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGCGCGCGGCGCGGCGCGG1996670.2752933361645758No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1021610.14085573738228765No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATCG1179950.069.489041
TCGCATC1033050.069.4665157
TCGATAG1195250.069.448255
CTGTATC844100.069.397384
ACTGTAT843700.069.389083
GTATCTC846150.069.329196
ATGGTCT1220200.069.291636
TATCTCT852050.069.274627
GGTATTC1223900.069.27196512
AATGGTC1221500.069.2635355
ACAATGG1215850.069.2568743
GCATCTT1033700.069.221429
AGGTCTT1196550.069.1630715
TAAGGTC1201300.069.11204513
CGCATCT1035850.069.1070258
ATACAGG1227250.069.029157
AACAATG1223050.069.0247042
GATACAG1227000.069.015886
CGATAGG1200650.069.007956
CAATGGT1222600.068.997574
Produced by FastQC (version 0.11.5)