FastQCFastQC Report
Tue 3 Jul 2018
HWLKJBGX5_n01_MYCScreens2_5_BC_bc9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWLKJBGX5_n01_MYCScreens2_5_BC_bc9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12283984
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATCG1829150.069.958811
TCGATTC2626000.069.850362
ATCGATT2624800.069.845651
TCGATAA4057150.069.80743
TAACAAT1871800.069.724121
GATAACG4055950.069.71935
ATAACGC4053250.069.7124946
TAACGCA4051350.069.698817
CGATAAC4063250.069.661544
AACGCAT4054000.069.6221548
ATTCCTT2633400.069.6039355
TCGATAG1840850.069.571245
ACGCATT4057300.069.5647669
TCGATAC1764150.069.53394
CGATTCC2636600.069.4845663
TTGGTTC2632500.069.3952710
CGCATTT4064450.069.3643110
TTCCTTG2642800.069.34876
GATTCCT2645650.069.314164
GGTTCTT2633600.069.31212
Produced by FastQC (version 0.11.5)