FastQCFastQC Report
Tue 3 Jul 2018
HWLKJBGX5_n01_MYCScreens2_5_BC_bc22.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWLKJBGX5_n01_MYCScreens2_5_BC_bc22.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11847280
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGCGCGCGGCGCGGCGC11041789.320097102457273No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGCGCGCGGCGC9838728.30462350851841No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGCGCGCGGCG9496368.015645785361704No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGCGCGCGGCGCGGCGCG9220367.7826809191645685No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGCGCGCGGCGCGGCGCGG9130637.706942015382435No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGCCAATACGGACCGGA3882883.277444274128745No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGCCAATACGG3379882.8528742462404875No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGCCAATACGGACCGGATT3330842.8114807787103873No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGCCAATACGGACCGGAT3248692.742139967992653No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGCCAATACGGA3141482.6516466226847006No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGGTAGCGCACGATATT2222161.87567104010372No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGGTAGCGCAC1948721.6448670074481233No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGGTAGCGCACGATATTAG1908431.6108592014369543No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGCTTTGGGGATCTCAA1871171.579408944500341No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGGTAGCGCACGATATTA1869481.5779824567326846No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGGTAGCGCACG1809751.5275658210154566No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGCTTTGGGGA1661171.4021530680459988No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGCTTTGGGGATCTCAAGA1608181.357425501887353No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGCTTTGGGGATCTCAAG1571441.3264141642638647No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGGATTCTGAGGGAAAT1509221.2738957802972497No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGCTTTGGGGAT1504141.269607876238259No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGGATTCTGAG1322511.1162984246172962No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGGATTCTGAGGGAAATTG1274151.0754789284966675No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGGATTCTGAGGGAAATT1243021.0492028550013168No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGGATTCTGAGG1218751.0287171401368078No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGCTGAAGGTTCCAGGT1147730.9687708908711536No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGCTGAAGGTT993860.8388929779662504No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGCTGAAGGTTCCAGGTCA979700.8269408674396149No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGCTGAAGGTTCCAGGTC958890.8093756541585917No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGCTGAAGGTTC939440.7929583836965108No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCTCT858140.7243350372406155No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGGAGACGGAC752330.635023397775692No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGGAGACGGACG744240.628194826154189No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCTCTG732390.618192530268551No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCTCTGT723570.6107477834574687No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGCTCCGCGCTTCTGCT235800.19903302699016148No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGCTCCGCGCT205100.17311990600374094No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGCTCCGCGCTT203640.1718875556245822No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGCTCCGCGCTTCTGCTT203300.1716005699198466No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGCTCCGCGCTTCTGCTTC199360.16827491204732226No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCATA500.070.003667
TCGCACT900.070.003667
TCGCAAT207.9296035E-570.003667
TCGTCTT700.070.003667
TCGTATC1300.070.003667
TCGAATC800.070.003667
TCGTTAC1350.070.002784
TCGTACA1600.070.002784
TCGATCA550.070.002774
GCATTTT252.8420309E-670.002489
CATTTTG252.8420309E-670.0024810
TCGGTCG301.024855E-770.002473
GTATTTT1500.070.00188413
ACGTTCG1550.070.00131
AACGCGA150.002225551869.99892430
CCCCGTA150.002225626369.9983437
TCGAGTC150.002225663569.9980541
ACGATCG2305200.069.9618151
TAACAAT2226600.069.955711
ATACTGT2207950.069.9030151
Produced by FastQC (version 0.11.5)