FastQCFastQC Report
Tue 3 Jul 2018
HWLKJBGX5_n01_MYCScreens2_5_BC_bc20.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWLKJBGX5_n01_MYCScreens2_5_BC_bc20.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16963020
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGCGCGCGGCGCGGCGC10940196.449435301025407No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGCGCGCGGCGC9079715.352649469257243No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGCGCGCGGCGCGGCGCGG8848645.216429621612189No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGCGCGCGGCGCGGCGCG8812255.194977073657875No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGCGCGCGGCG8694945.125820755973877No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGCCAATACGGACCGGA1570290.9257136995652896No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGCCAATACGGACCGGATT1341810.7910207026814801No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGCCAATACGGA1328580.7832213839280977No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGCCAATACGG1282710.7561802084770283No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGCCAATACGGACCGGAT1262820.7444547020518751No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGGTAGCGCACGATATT968480.5709360715249997No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGCTTTGGGGATCTCAA946210.5578075130489736No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGGTAGCGCACGATATTAG827780.48799093557633016No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGGTAGCGCACG826780.4874014179078961No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGCTTTGGGGATCTCAAGA816100.48110536920902053No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGCTTTGGGGAT804900.4745027713225593No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGGTAGCGCAC796110.4693209110170241No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGGTAGCGCACGATATTA784570.46251787712329523No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGCTTTGGGGA777830.4585445280380498No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGCTTTGGGGATCTCAAG770180.45403471787452937No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGCTGAAGGTTCCAGGT732260.4316802078875106No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGCTGAAGGTTC617840.36422759626528767No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGCTGAAGGTTCCAGGTCA614640.3623411397262987No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGCTGAAGGTT595350.3509693439022061No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGCTGAAGGTTCCAGGTC592790.349460178671015No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCTCT396130.23352563399677653No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGGATTCTGAGGGAAAT389010.22932826819752616No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGGCCGCCACTGGAGGG341750.2014676631873334No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGGAGACGGACG330480.19482379906408173No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGGATTCTGAGG328530.19367423961063537No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCTCTGT325780.19205306602244177No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGGATTCTGAGGGAAATTG323680.19081507891873026No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCTCTG317000.1868771008935909No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGGATTCTGAG316720.18671203594642935No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGGAGACGGAC316020.18629937357852552No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGGATTCTGAGGGAAATT306900.18092297244240707No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGGCCGCCACTG286730.1690324010700925No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGGCCGCCACTGGAGGGCG277530.16360883852049932No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGGCCGCCACTGGAGGGC276540.1630252160287496No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGGCCGCCACT275830.16260665848416145No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATCG3140450.069.9599461
TAACAAT3224750.069.840521
TACTGTA3130500.069.777432
TAGGTCG3916800.069.771373
ATAGGTC3919250.069.769522
AGGTCGC3918050.069.708224
TCGATAC3306550.069.677724
ACTGTAT3135100.069.661853
CTGTATC3135650.069.638684
TCGATAG3157200.069.633745
GGTCGCA3922200.069.621625
ATACTGT3138050.069.619741
GTATCTC3137050.069.5833746
TGTATCT3139350.069.5703055
GTCGCAT3926100.069.5578166
TCGCATC3926350.069.543987
AACAATG3252200.069.529772
TATCTCT3142900.069.527777
ATCTCTT3144350.069.4362958
CGATAGG3163050.069.400946
Produced by FastQC (version 0.11.5)