FastQCFastQC Report
Tue 3 Jul 2018
HWLKJBGX5_n01_MYCScreens2_5_BC_bc18.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWLKJBGX5_n01_MYCScreens2_5_BC_bc18.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12201819
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGCGCGCGGCGCGGCGC139920.11467142726834416No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGCGCGCGGCGC122490.10038667185605686No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGCGCGCGGCG122490.10038667185605686No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATCG2155200.069.952051
TAGGTCG2716900.069.76243
TAACAAT2371250.069.761511
TCGATAC2417500.069.746324
AGGTCGC2717450.069.716654
ATAGGTC2720350.069.679072
GGTCGCA2719850.069.6298145
TACTGTA2372850.069.609662
TCGATAG2168750.069.583075
GTCGCAT2723150.069.568576
TCGCATC2723900.069.549287
CTGTATC2373900.069.542574
ACTGTAT2375150.069.515723
GTATCTC2376800.069.431446
TGTATCT2379100.069.42175
TATCTCT2381800.069.354437
ATACTGT2382850.069.35141
AACAATG2393250.069.3468252
ATCTCTT2382600.069.2367868
AATGGTC2394650.069.2076955
Produced by FastQC (version 0.11.5)