FastQCFastQC Report
Tue 3 Jul 2018
HWLKJBGX5_n01_MYCScreens2_5_BC_bc17.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWLKJBGX5_n01_MYCScreens2_5_BC_bc17.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11976959
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGCGCGCGGCGCGGCGC298650.249353780037153No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGCGCGCGGCGCGGCGCG274530.22921511211652307No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGCGCGCGGCGCGGCGCGG270630.22595885984079933No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGCGCGCGGCG269850.22530760938565458No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGCGCGCGGCGC231450.19324604851698998No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATCG2323750.069.949721
TAACAAT2466650.069.79511
TAGGTCG2636500.069.752153
AGGTCGC2635850.069.727734
ATAGGTC2642000.069.682462
TCGATAC1923100.069.664924
GGTCGCA2639300.069.637755
TACTGTA2463650.069.630742
TCGATAG2336300.069.630395
CTGTATC2463800.069.579074
GTCGCAT2642050.069.5771946
ACTGTAT2464950.069.5727163
TCGCATC2642050.069.5549557
TGTATCT2469250.069.4555
GTATCTC2467850.069.451866
AACAATG2488050.069.410722
ATACTGT2472750.069.370991
TATCTCT2473550.069.362867
ATCTCTT2471950.069.339248
GCATCTT2652650.069.2729649
Produced by FastQC (version 0.11.5)