FastQCFastQC Report
Tue 3 Jul 2018
HWLKJBGX5_n01_MYCScreens2_5_BC_bc14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWLKJBGX5_n01_MYCScreens2_5_BC_bc14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12240785
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGCGCGCGGCGCGGCGC908590.7422644871223537No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGCGCGCGGCGCGGCGCG806120.6585525356421177No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGCGCGCGGCGCGGCGCGG788970.6445419962853689No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGCGCGCGGCG787040.6429653000195658No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGCGCGCGGCGC780030.6372385431163116No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGCTGAAGGTTCCAGGT378860.30950629391824136No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGCTGAAGGTTCCAGGTC353670.2889275483557631No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGCTGAAGGTTCCAGGTCA349340.2853901935210854No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGCTGAAGGTTC345510.28226130922159No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGCTGAAGGTT340040.2777926415666969No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGCCAATACGGACCGGA173250.14153504044062534No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGCCAATACGGACCGGATT162660.13288363450546678No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGCCAATACGGACCGGAT161350.13181344170328946No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGCCAATACGGA155330.126895456459696No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGCCAATACGG152830.12485310378378511No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATCG2316300.069.956271
TAACAAT2396250.069.786841
TAGGTCG2616150.069.775593
TCGATAC2341550.069.7326054
AGGTCGC2615900.069.7276844
ATAGGTC2620650.069.695852
TCGATAG2329050.069.65455
TACTGTA2406400.069.646562
GGTCGCA2618550.069.646435
CTGTATC2407600.069.6048664
GTCGCAT2621100.069.59876
TCGCATC2621300.069.571267
ACTGTAT2409100.069.564153
TGTATCT2412500.069.456235
ATACTGT2413450.069.427251
GTATCTC2412150.069.4227756
TATCTCT2417200.069.3405767
AACAATG2419650.069.336072
AATGGTC2419350.069.278385
GCATCTT2631150.069.273799
Produced by FastQC (version 0.11.5)