FastQCFastQC Report
Tue 3 Jul 2018
HWLKJBGX5_n01_MYCScreens2_5_BC_bc12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWLKJBGX5_n01_MYCScreens2_5_BC_bc12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12184308
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGTCG455.456968E-1269.999431
ACGATCG2516800.069.9549261
TCGATTC2557850.069.843852
ATCGATT2558250.069.832521
TAACAAT2387400.069.780981
TCGATAA2431300.069.694793
TCGATAG2531950.069.6189045
GATAACG2429850.069.6170965
ATTCCTT2563200.069.600395
TAACGCA2428600.069.591817
ATAACGC2430300.069.575416
TCGATAC2145250.069.567034
CGATTCC2564850.069.536163
AACGCAT2431000.069.523988
CGATAAC2436000.069.511464
ACGCATT2432150.069.478479
TTGGTTC2562300.069.38457510
AACAATG2409450.069.364872
GATTCCT2573450.069.3541644
TTCCTTG2572200.069.326926
Produced by FastQC (version 0.11.5)