FastQCFastQC Report
Tue 3 Jul 2018
HWLKJBGX5_n01_MYCScreens2_5_BC_bc11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWLKJBGX5_n01_MYCScreens2_5_BC_bc11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9876623
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGTCG455.456968E-1269.9995651
ACGATCG1866450.069.962071
TCGATTC1580600.069.860952
TCGATAA2821150.069.801413
TAACAAT1838200.069.7863161
ATCGATT1582150.069.769511
GATAACG2820700.069.7070545
TAACGCA2818100.069.6780247
ATAACGC2820300.069.677236
CGATAAC2824700.069.669244
TCGATAG1876100.069.641685
AACGCAT2820200.069.6030968
TCGATAC1653750.069.595684
ACGCATT2822750.069.548939
ATTCCTT1585450.069.5437245
CGATTCC1589250.069.3749853
AACAATG1858200.069.31172
CGCATTT2829400.069.3041810
TTGGTTC1583500.069.29137410
GGTTCTT1583000.069.26250512
Produced by FastQC (version 0.11.5)