FastQCFastQC Report
Tue 3 Jul 2018
HWLKJBGX5_n01_MYCScreens2_5_BC_bc10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWLKJBGX5_n01_MYCScreens2_5_BC_bc10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14325078
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTC207.9325175E-569.9985736
ACGATCG2413150.069.9553151
TCGATTC3256600.069.8286062
ATCGATT3257000.069.8208161
TCGATAA3837050.069.7940753
TAACAAT2468500.069.726631
ATAACGC3835550.069.679626
GATAACG3838250.069.6725545
TAACGCA3834950.069.652187
CGATAAC3844750.069.614064
TCGATAG2428150.069.613315
ATTCCTT3263000.069.603145
AACGCAT3838350.069.589338
TCGATAC2186000.069.582594
CGATTCC3265800.069.5421143
ACGCATT3840900.069.539619
GATTCCT3274850.069.3955464
TTGGTTC3262950.069.390710
TTCCTTG3273400.069.371316
GGTTCTT3263500.069.3140312
Produced by FastQC (version 0.11.5)