FastQCFastQC Report
Wed 1 Aug 2018
HWCMHBGX5_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCMHBGX5_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14259623
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG265100.18590954333084403No Hit
GGGGAGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG164610.11543783450656445No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGTCT12750.043.617814
CTGAACT14350.043.14134219
ACGTCTG13400.042.28462215
ACACGTC14800.038.0489413
GAACTCC17000.035.388421
CAGGCCT25050.030.02009821
TATCTCG10850.027.0844045
CTTGTCT10750.026.6565071
GGATATC11400.026.1461982
ATTGAAC40550.023.5530766
GATATCT12700.023.1478063
AGGCCTA33150.022.7905422
GGGGAGG56350.022.7897421
CCTAACA34250.022.6748225
ATATCTC14050.022.1624054
GCCTAGG35300.021.812150
GAACGGT26750.021.06341442
ACATGCC14550.020.4376876
CGGTAAC26300.019.96434845
CTAACAC39900.019.55165126
Produced by FastQC (version 0.11.5)