FastQCFastQC Report
Wed 1 Aug 2018
HWCMHBGX5_n01_9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCMHBGX5_n01_9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences380973424
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCG8025870.21066745065136092No Hit
CTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAA6193780.16257774453054763No Hit
CTTTTATCCGTTGAGCGATGGCCCTTCCATTCAGAACCACCGGATCACTA4073680.10692819350044742No Hit
CGACTGTTTACCAAAAACACAGGTCTCCGCAAAGTCGTAAGACCATGTAT3896280.10227170071579587No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG410300.057.8352343
CTCGTAT363400.055.91860242
GTATGCC422050.055.79384645
TATGCCG428850.054.23990646
TCAGATT1275600.052.126152
CAGATTG1328500.050.132213
AGATTGA1351150.049.5690234
TTGAACG1350300.049.1351787
ATGCCGT475450.048.18766847
GCCGTCT419100.047.7184349
TGCCGTC484200.044.8664548
CACGTCT628100.044.2055214
ACGTCTG635550.043.67609415
TAATCTC488850.043.10046438
CTGAACT655100.042.44182619
GATTGAA1601150.041.6457255
GAACTCC660900.041.6298521
ACACGTC675200.041.236513
CGTATGC578000.040.77039744
CTTGTCT577350.040.7646941
Produced by FastQC (version 0.11.5)