FastQCFastQC Report
Wed 1 Aug 2018
HWCMHBGX5_n01_8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCMHBGX5_n01_8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26610553
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGGCTCTCGCATCGATGAAGAACGTAGCGAAATGCGATACCTGGTGTG351190.13197395785048133No Hit
CTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTATGAACTAATTCGAAC315900.11871230184506124No Hit
CGACTGTTTACCAAAAACACAGGTCTCCGCAAAGTCGTAAGACCATGTAT308850.1160629769700765No Hit
CGGATATCTCGGCTCTCGCATCGATGAAGAACGTAGCGAAATGCGATACC293970.11047121042542783No Hit
GAACAATCCAACACTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCG285480.10728074685257387No Hit
AGAACATCTAAGGGCATCACAGACCTGTTATTGCCTCAAACTTCCGTGGC269750.10136955815987739No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG27650.044.4299946
TCGTATG30900.039.6436643
GTATGCC32500.038.01505345
CTCGTAT29450.036.8417842
CACGTCT38400.036.45849214
ACACGTC40550.035.12995513
ACGTCTG40300.034.65275615
TATCTCG75050.032.086445
CGTATGC39500.031.36681444
ATGCCGT38900.031.31078147
GGATATC80150.030.9182132
CTTGTCT83200.029.4908641
ATATCTC89450.027.7036864
TGTTTAC82100.027.4126915
GATATCT91150.027.2253973
GCCGTCT41950.027.19875749
AGTCACC62100.025.13750628
TGCCGTC47600.024.70566648
CGGGACT61950.024.0691681
TCTCGGC103150.023.650357
Produced by FastQC (version 0.11.5)