FastQCFastQC Report
Wed 1 Aug 2018
HWCMHBGX5_n01_7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCMHBGX5_n01_7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17017959
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCG204590.1202200569410233No Hit
CTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAA181710.10677543646685245No Hit
CTCGGCTCTCGCATCGATGAAGAACGTAGCGAAATGCGATACCTGGTGTG179600.10553556980599141No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCACAT30950.054.16907529
ACATTCC31550.052.91626732
CTCGTAT17100.052.8071244
GTATGCC17300.052.80357447
TCGTATG16000.052.71885345
TATGCCG17700.050.6215748
CACATTC34050.049.8535531
CACGTCT34500.049.50734314
ACGTCTG35450.048.57541315
ACACGTC36150.047.1507213
CTGAACT37250.046.50961719
GAACTCC37450.044.9525721
AGTCACA40750.042.77384628
TCAGATT38550.042.673072
GCCGTCT16250.040.92291651
GAGCACA42400.040.613419
CAGATTG41850.039.14093
ATGCCGT21300.038.77919449
AGATTGA44050.037.5040134
TTGAACG44000.036.670427
Produced by FastQC (version 0.11.5)