FastQCFastQC Report
Wed 1 Aug 2018
HWCMHBGX5_n01_6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCMHBGX5_n01_6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10048029
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCACAT31700.059.5111429
CACGTCT32600.058.8347414
ACACGTC33600.057.5018113
ACGTCTG33550.057.3771415
CTGAACT35300.054.5321219
ACATGAG34800.054.4101932
CACATGA35150.054.26669731
GAACTCC35900.053.71821221
TGAGCAT36000.049.68013835
GAGCACA39200.049.5553749
AGTCACA40750.047.92645628
GAGCATC33650.043.0605636
TCTAGTT26850.040.6720737
GTACTCT27250.040.3319443
TAGTTGT29250.037.3346949
TCTAATG30250.035.52036320
TACCTCT30400.035.34562716
CAGTCAC64150.034.4817427
TCTGAAC56600.034.44368718
AACTCCA58800.033.9879822
Produced by FastQC (version 0.11.5)