FastQCFastQC Report
Wed 1 Aug 2018
HWCMHBGX5_n01_5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCMHBGX5_n01_5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9309875
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTAT648790.696883685334121TruSeq Adapter, Index 25 (97% over 44bp)
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA234340.25171122061252166No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT108540.11658588326910942No Hit
CTGTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATG94760.10178439560144471No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG75250.066.55704545
CTCGTAT71900.065.8612344
TATGCCG74850.065.6505848
GTATGCC75350.065.6329947
TGATATA79300.064.4377836
ATGCCGT76450.064.2308149
GCCGTCT74500.063.9858751
TGCCGTC76550.063.50680550
GATATAT80450.063.47316737
TATATCT75850.062.9851239
ACTGATA81750.062.93609634
CACGTCT82900.062.8648814
ACGTCTG83150.062.633115
CGTATGC80600.062.0089146
GAACTCC83650.061.75730521
CTGAACT84550.061.67912319
ACACGTC85050.061.5654213
ATATATC79300.060.68626838
GCTTGAA75150.059.47340861
AGTCACA87550.059.4472928
Produced by FastQC (version 0.11.5)