FastQCFastQC Report
Wed 1 Aug 2018
HWCMHBGX5_n01_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCMHBGX5_n01_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20629608
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAACAATCCAACACTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCG231580.11225613206028928No Hit
CTCGGCTCTCGCATCGATGAAGAACGTAGCGAAATGCGATACCTGGTGTG229060.11103458679389351No Hit
CGACTGTTTACCAAAAACACAGGTCTCCGCAAAGTCGTAAGACCATGTAT224210.10868359689626676No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA219810.10655074008192499No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCACGT18650.051.98563829
CACGTTT18850.051.43294531
AGTCACG18850.051.24851228
ACGTTTC20550.047.17815432
CGTTTCG20850.046.33135233
TTCGGAA21850.043.88959536
ACGTCTG24400.040.88101615
CACGTCT24800.040.50439514
ACACGTC26150.038.54775213
CTGAACT27250.036.21989419
TCAGATT25550.033.7000772
GAACTCC29150.033.61886221
TATCTCG44500.033.585635
GGATATC47550.031.652252
ATATCTC47700.031.6260194
GAGCACA33650.030.3720389
CAGATTG29300.029.7453023
GATATCT52750.028.9965063
CTTGTCT49600.028.2979261
GGCAACG20150.028.1401632
Produced by FastQC (version 0.11.5)