FastQCFastQC Report
Wed 1 Aug 2018
HWCMHBGX5_n01_16.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCMHBGX5_n01_16.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14645110
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAACAATCCAACACTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCG166280.11353960468716177No Hit
CTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTATGAACTAATTCGAAC154120.10523649190753773No Hit
CGACTGTTTACCAAAAACACAGGTCTCCGCAAAGTCGTAAGACCATGTAT150130.10251203302672358No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGTCT30100.055.349414
ACGTCTG31750.052.80314615
ACACGTC32150.051.82100313
GAATATC30650.049.4450136
CTGAACT35300.047.49258419
AATATCG24350.045.42047537
GAACTCC37400.044.35828421
GAGCACA37800.044.3532149
GTCACCG37150.043.8090329
ATATCGG18450.042.30308538
TCAGATT28400.036.726352
AGTCACC55850.034.02887728
CAGATTG30650.033.80193
TTGAACG30050.033.777847
TATCTCG24800.032.1781855
AGATTGA34450.031.2926884
CAGTCAC61900.031.04227
GGATATC26650.030.7325462
GTCTGAA57600.029.7135417
CTTGTCT44500.029.0238571
Produced by FastQC (version 0.11.5)